|
KMID : 0578320060210030343
|
|
Molecules and Cells
2006 Volume.21 No. 3 p.343 ~ p.355
|
|
|
Profiling of Differentially Expressed Genes in Human Stem Cells by cDNA Microarray
|
|
Kim Chul-Geun
Lee Jong-Joo
Jung Dae-Young
Jeon Jin-Seon
Heo Hyen-Seok
Kang Ho-Chul
Shin June-Ho
Cho Yoon-Shin
Cha Kyung-Joon
Kim Chan-Gil
Do Byung-Rok
Kim Kyung-Suk
Kim Hyun-Soo
|
|
|
Abstract
|
|
|
|
Stem cells are unique cell populations with the ability to undergo both self-renewal and differentiation, although a wide variety of adult stem cells as well as embryonic stem cells have been identified and stem cell plasticity has recently been reported. To identify genes implicated in the control of the stem cell state as well as the characteristics of each stem cell line, we analyzed the expression profiles of genes in human embryonic, hematopoietic (CD34+ and CD133+), and mesenchymal stem cells using cDNA microarrays, and identified genes that were differentially expressed in specific stem cell populations. In particular we were able to identify potential hESC signature-like genes that encode transcription factors (TFAP2C and MYCN), an RNA binding protein (IMP-3), and a functionally uncharacterized protein (MAGEA4). The overlapping sets of 22 up-regulated and 141 down-regulated genes identified in this study of three human stem cell types may also provide insight into the developmental mechanisms common to all human stem cells. Furthermore, our comprehensive analyses of gene expression profiles in various adult stem cells may help to identify the genetic pathways involved in self-renewal as well as in multi-lineage specific differentiation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|