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KMID : 0578320060220010065
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Molecules and Cells
2006 Volume.22 No. 1 p.65 ~ p.69
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Involvement of the Phospholipase C b1 Pathway in Desensitization of the Carbachol-activated Nonselective Cationic Current in Murine Gastric Myocytes
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Kim Byung-Joo
So In-Suk
Kim Ki-Whan
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Abstract
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In murine gastrointestinal myocytes muscarinic stimulation activates nonselective cation channels via a G-protein and Ca2+-dependent pathway. We recorded inward cationic currents following application of carbachol (ICCh) to murine gastric myocytes held at -60 mV, using the whole-cell patch-clamp method. The properties of the inward cationic currents were similar to those of the nonselective cation channels activated by muscarinic stimulation in other gastrointestinal smooth muscle cells. CCh-induced ICCh and spontaneous decay of ICCh (desensitization of ICCh) occurred. Unlike the situation in guinea pig gastric myocytes, desensitization was not affected by varying [EGTA]i. Pretreatment with the PLC inhibitor (U73122) blocked the activation of ICCh, and desensitization of ICCh was attenuated in PLC b1 knock-out mice. These results suggest that the desensitization of ICCh in murine gastric myocytes is not due to a pathway dependent on intracellular Ca2+ but to the PLC b1 pathway.
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KEYWORD
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Carbachol (CCh), Desensitization, Nonselective Cationic Current, PLC β1, Smooth Muscle
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