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KMID : 0578320060220010089
Molecules and Cells
2006 Volume.22 No. 1 p.89 ~ p.96
Molecular Cloning of a Pepper Gene that Is Homologous to SELF-PRUNING
Kim Dong-Hwan

Han Myeong-Suk
Kim Byung-Dong
Cho Hyun-Wooh
Jo Yeong-Deuk
Cho Myeong-Cheoul
Abstract
¡°Determinate¡± and ¡°indeterminate¡± inflorescences in plants are controlled by a single recessive gene, for example, SELF-PRUNING (SP) in Solanum lycopersicum, TERMINAL FLOWER1 in Arabidopsis, CENTRORADI-ALIS in Antirrhinum, and CENTRORADIALIS-like gene in tobacco. Pepper (Capsicum annuum L.) is an indeterminate species in which shoots grow indefinitely. In this study, we cloned and characterized the pepper SP-like gene (CaSP). RT-PCR revealed that the CaSP transcript accumulates to higher levels in floral buds than in other organs. Comparison of genomic DNA and cDNA sequences from indeterminate and determinate pepper plants revealed the insertion of a single base in the first exon of CaSP in the determinate pepper plants. CaSP is annotated in linkage group 8 (chromosome 6) of the SNU2 pepper genetic map and showed similar synteny to SP in tomato. Transgenic tobacco plants overexpressing CaSP displayed late-flowering phenotypes similar to the phenotypes caused by overexpression of CaSP orthologs in other plants. Collectively, these results suggest that pepper CaSP is an ortholog of SP in tomato.
KEYWORD
Capsicum annuum L., Determinate Inflorescence, Linkage Map, Phylogenic Tree, SELF-PRUNING(SP)
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