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KMID : 0578320060220020141
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Molecules and Cells
2006 Volume.22 No. 2 p.141 ~ p.145
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Mouse Transthyretin-related Protein Is a Hydrolase which Degrades 5-Hydroxyisourate, the End Product of the Uricase Reaction
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Lee You-Ra
Park Byoung-Chul
Lee Doo-Hee
Bae Kwang-Hee
Park Sung-Goo
Cho Sa-Yeon
Lee Choong-Hwan
Lee Jong-Suk
Myung Pyung-Keun
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Abstract
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Uric acid is the end product of the purine degradation pathway in humans. It is catabolized to allantoin by urate oxidase or uricase (E.C. 1.7.3.3.) in most vertebrates except humans, some primates, birds, and certain species of reptiles. Here we provide evidence that mouse transthyretin-related protein facilitates the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction. Mutagenesis experiments showed that the residues that are absolutely conserved across the TRP family, including His11, Arg51, His102, and the C-terminal Tyr-Arg-Gly-Ser, may constitute the active site of mTRP. Based on these results, we propose that the transthyretin-related proteins present in diverse organisms are not functionally related to transthyretin but actually function as hydroxyisourate hydrolases.
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KEYWORD
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Hydroxyisourate, Transthyretin-related Proteins, Uric Acid, Uricase
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