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KMID : 0578320060220020154
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Molecules and Cells
2006 Volume.22 No. 2 p.154 ~ p.162
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Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: Cloning, Sequencing, and Characterization
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Basnet Devi Bahadur
Oh Tae-Jin
Vu Thi Thu Hang
Sthapit Basundhara
Liou Kwangkyoung
Lee Hei-Chan
Sohng Jae-Kyung
Yoo Jin-Cheol
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Abstract
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The entire gene cluster involved in the biosynthesis of angucyclines Sch 47554 and Sch 47555 was cloned, sequenced, and characterized. Analysis of the nucleotide sequence of genomic DNA spanning 77.5-kb revealed a total of 55 open reading frames, and the deduced products exhibited strong sequence similarities to type II polyketide synthases, deoxysugar biosynthetic enzymes, and a variety of accessory enzymes. The involvement of this gene cluster in the pathway of Sch 47554 and Sch 47555 was confirmed by genetic inactivation of the aromatase, including a portion of the ketoreductase, which was disrupted by inserting the thiostrepton gene.
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KEYWORD
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Aculose, Amicetose, Angucycline, Polyketide, Sch 47554, Sch 47555, Streptomyces
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