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KMID : 0578320070230010094
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Molecules and Cells
2007 Volume.23 No. 1 p.94 ~ p.99
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PMA Activates Stat3 in the Jak/Stat Pathway and Induces SOCS5 in Rat Brain Astrocytes
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Hwang Mi-Na
Kim Kwang-Soo
Choi Yo-Woo
Jou Ilo
Yoon Sung-Pil
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Abstract
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Suppressors of cytokine signaling (SOCS) family members are negative feedback regulators of the Jak/Stat pathway, which is an essential inflammatory signaling pathway. We investigated expression of eight members of the SOCS family in rat astrocytes, using two inflammatory stimulants, PMA and IFN-?. Only a few SOCS genes were induced by both stimulants, and we detected an increase in SOCS5 protein with PMA. PMA activated the Jnk, Erk, p38, and Jak/Stat signal pathways. In addition, it increased the level of activated-Stat3 resulting from tyrosine phosphorylation. A gel-shift assay showed that a protein in nuclear extracts from PMA-treated cells was able to bind to Stat binding elements. These results suggest that activated Stat3 binds to SOCS promoters and leads to their transcriptional induction.
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KEYWORD
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Astrocyte, IFN-γ, Jak/Stat, PMA, SOCS, Stat3
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