KMID : 0578320070230020170
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Molecules and Cells 2007 Volume.23 No. 2 p.170 ~ p.174
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Identification of Novel Regulators of Apoptosis Using a High-Throughput Cell-based Screen
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Park Kyung-Mi
Kang Eun-Ju Jeon Yeo-Jin Kim Na-Young Kim Nam-Soon Yoo Hyang-Sook Yeom Young-Il Kim Soo-Jung
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Abstract
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High-throughput subcellular imaging is a powerful tool for investigating the function of genes. In order to identify novel regulators of apoptosis we transiently transfected HeLa cells with 938 hypothetical genes of unknown function, and captured their nuclear images with an automated fluorescence microscope. We selected genes that induced greater than 3-fold increase in the percentage of apoptotic nuclei compared with vector-transfected cells. The full-length genes C10orf61, MGC 26717, and FLJ13855 were identified as candidate proapoptotic genes, and their apoptotic effects were confirmed by DNA fragmentation ELISAs and Western blotting for caspase-7 and PARP. We conclude that a subcellular image-based apoptotic screen is useful for identifying genes with proapoptotic activity.
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KEYWORD
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Apoptosis, Automated Fluorescence Microscope, Hypothetical Genes, Subcellular Imaging
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