|
KMID : 0578320070230030316
|
|
Molecules and Cells
2007 Volume.23 No. 3 p.316 ~ p.322
|
|
|
ZNF435, a Novel Human SCAN-containing Zinc Finger Protein, Inhibits AP-1-mediated Transcriptional Activation
|
|
Gu Xing
Zheng Mei
Fei Xiangwei
Yang Zhenxing
Ji Chaoneng
Xie Yi
Mao Yumin
|
|
|
Abstract
|
|
|
|
Zinc finger transcription factor genes are a significant fraction of the genes in the vertebrate genome. Here we report the isolation and characterization of a human zinc finger-containing gene, ZNF435, from a fetal brain cDNA library. ZNF435 cDNA is 1290 base pairs in length and contains an open reading frame encoding 349 amino acids with four C2H2-type zinc fingers at its carboxyl terminus and a SCAN motif at its amino terminus. RT-PCR results showed that ZNF435 was expressed in all tested tissues. A ZNF435-GFP fusion protein was located in the nucleus and the four zinc fingers acted as nuclear localization signals (NLSs). ZNF435 was found to be capable of homo-association, and this effect was independent of its zinc fingers. Furthermore, ZNF435 proved to be a transcription repressor as its overexpression in AD293 cells inhibited the transcriptional activities of AP-1
|
|
|
KEYWORD
|
|
|
AP-1, C2H2 Zinc Finger Protein, Homo-Association, MAPK Signaling Pathway, SCAN Domain, Transcriptional Suppressor.
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|