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KMID : 0578320070230030398
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Molecules and Cells
2007 Volume.23 No. 3 p.398 ~ p.404
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Methanol Extracts of Stewartia koreana Inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by Blocking NF-κB Transactivation in LPS-activated RAW 264.7 Cells
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Lee Tae-Hoon
Kwak Han-Bok
Kim Hong-Hee
Lee Zang-Hee
Chung Dae-Kyun
Baek Nam-In
Kim Ji-Young
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Abstract
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Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are involved in various pathophysiological processes such as inflammation and carcinogenesis. In a search for inhibitors of COX-2 and iNOS production we found that extracts of Stewartia koreana strongly inhibited NO and PGE2 production in LPS-treated macrophage RAW 264.7 cells. We have now shown that the mRNA and protein levels of iNOS and COX-2 are reduced by the Stewartia koreana extract (SKE). SKE inhibited expression of an NF-?B reporter gene in response to LPS, and gel mobility shift assays revealed that SKE reduced NF-?B DNA-binding activity. The extract also inhibited LPS-induced phosphorylation of I?B-? and nuclear translocation of p65. Administration of the extract reduced the symptoms of arthritis in a collagen-induced arthritic mouse model. These results indicate that Stewartia extracts contain potentially useful agents for preventing and treating inflammatory diseases
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KEYWORD
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Cyclooxygenase-2, Inflammation, Nitric Oxide Synthase, Nuclear factor κB, Stewartia koreana
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