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KMID : 0578320070230030405
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Molecules and Cells
2007 Volume.23 No. 3 p.405 ~ p.409
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Expression of a Functional Type-I Chalcone Isomerase Gene Is Localized to the Infected Cells of Root Nodules of Elaeagnus umbellata
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Kim Ho-Bang
Bae Ju-Hee
Lim Jung-Dae
Yu Chang-Yeon
An Chung-Sun
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Abstract
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A putative type-I chalcone isomerase (CHI) cDNA clone EuNOD-CHI was previously isolated from the root nodule of Elaeagnus umbellata [Kim et al. (2003)]. To see if it encodes a functional CHI, we ectopically overexpressed it in the Arabidopsis (Arabidopsis thaliana) transparent testa 5 (tt5) mutant, which is defective in naringenin production and has yellow seeds due to proanthocyanidin deficiency. Ectopic overexpression of EuNOD-CHI resulted in recovery of normal seed coat color. Naringenin produced by CHI from naringenin chalcone was detected in the transgenic lines like in the wild-type, whereas it was absent from the tt5 mutant. We conclude that EuNOD-CHI encodes a functional type-I CHI. In situ hybridization revealed that EuNOD-CHI expression is localized to the infected cells of the fixation zone in root nodules
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KEYWORD
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Arabidopsis tt5 Mutant, Chalcone Isomerase, Molecular Complementation, Elaeagnus umbellata, Flavonoids Analysis, Frankia, In Situ Hybridization, Root Nodule
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