KMID : 0578320070240010113
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Molecules and Cells 2007 Volume.24 No. 1 p.113 ~ p.118
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Ameliorating Effect of Gardenia jasminoides Extract on Amyloid Beta Peptide-induced Neuronal Cell Deficit
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Choi Soo-Jung
Kim Mi-Jeong Heo Ho-Jin Hong Bum-Shik Cho Hong-Yon Kim Young-Jun Kim Hye-Kyung Lim Seung-Taik Jun Woo-Jin Shin Dong-Hoon
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Abstract
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The brains of Alzheimer¡¯s disease (AD) patients are characterized by large deposits of amyloid beta peptide (A¥â). A¥â is known to increase free radical production in nerve cells, leading to cell death that is characterized by lipid peroxidation, free radical formation, protein oxi-dation, and DNA/RNA oxidation. In this study, we selected an extract of Gardenia jasminoides by screening, and investigated its ameliorating effects on A¥â-induced oxidative stress using PC12 cells. The effects of the extract were evaluated using the 2?,7?-dichlorofluorescein diacetate (DCF-DA) assay and the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. To find the active component, the ethanol extract was partitioned with hexane, chloroform, and ethyl acetate, respectively, and the active component was purified by silica-gel column chromatography and HPLC. The results suggested that Gardenia jasminoides extract can reduce the cytotoxicity of A¥â in PC 12 cells, possibly by reducing oxidative stress
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KEYWORD
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Alzheimer¡¯s Disease, Amyloid Beta, Gardenia jasminoides, Oxidative Stress, PC12 Cells
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