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KMID : 0578320070240020343
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Molecules and Cells
2007 Volume.24 No. 2 p.343 ~ p.350
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In Vitro Differentiation of Mesenchymal Progenitor Cells Derived from Porcine Umbilical Cord Blood
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Kumar Basavarajappa Mohana
Yoo Jae-Gyu
Oak Sun-A
Kim Jung-Gon
Song Hye-Jin
Kang Eun-Ju
Cho Seong-Keun
Lee Sung-Lim
Cho Jae-Hyeon
Balasubramanian Sivasankaran
Rho Gyu-Jin
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Abstract
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Mesenchymal stem/progenitor cells (MPCs) were isolated from porcine umbilical cord blood (UCB) and their morphology, proliferation, cell cycle status, cell-surface antigen profile and expression of hematopoietic cytokines were characterized. Their capacity to differentiate in vitro into osteocytes, adipocytes and chondrocytes was also evaluated. Primary cultures of adherent porcine MPCs (pMPCs) exhibited a typical fibroblast-like morphology with significant renewal capacity and proliferative ability. Subsequent robust cell growth was indicated by the high percentage of quiescent (G0/G1) cells. The cells expressed the mesenchymal surface markers, CD29, CD49b and CD105, but not the hematopoietic markers, CD45 and CD133 and synthesized hematopoietic cytokines. Over 21 days of induction, the cells differentiated into osteocytes adipocytes and chondrocytes. The expression of lineage specific genes was gradually upregulated during osteogenesis, adipogenesis and chondrogenesis. We conclude that porcine umbilical cord blood contains a population of MPCs capable of self-renewal and of differentiating in vitro into three classical mesenchymal lineages.
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KEYWORD
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Mesenchymal Progenitor Cells, In vitro Differentiation, Porcine, Umbilical Cord Blood
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