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KMID : 0578320070240030372
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Molecules and Cells
2007 Volume.24 No. 3 p.372 ~ p.377
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EID-1 Interacts with Orphan Nuclear Receptor SF-1 and Represses Its Transactivation
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Park Yun-Yong
Park Ki-Cheol
Shong Min-Ho
Choi Hueng-Sik
Lee Soon-Jung
Lee Young-Ho
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Abstract
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The orphan nuclear receptor, SF-1, plays a pivotal role in the development and differentiation of the endocrine and reproductive systems, and also regulates the transcription of a host of genes, including those encoding several steroidogenic enzymes and gonadotropins. We found that a previously unidentified repressor, EID-1, is an SF-1-interacting protein that inhibits the transactivation of SF-1. A transient transfection assay revealed that EID-1 inhibits SF-1, but not LRH-1, ERR?, or mCAR. Using the yeast two hybrid and GST pull-down assays, we determined that EID-1 interacted strongly with SF-1. In addition, it colocalized with SF-1 in mammalian cells and interacted specifically with the AF-2 domain of SF-1, competing with SRC-1 to inhibit SF-1 transactivation. EID-1 is expressed in the mouse testis, and its expression decreases during testis development. The results of the present study suggest that EID-1 can act as a repressor, regulating the function of SF-1.
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KEYWORD
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EID-1, Nuclear Receptor, SF-1, Testis, Transcriptional Repression
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