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KMID : 0578320080250020272
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Molecules and Cells
2008 Volume.25 No. 2 p.272 ~ p.278
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Growth Retardation and Death of Rice Plants Irradiated with Carbon Ion Beams Is Preceded by Very Early Dose- and Time-dependent Gene Expression Changes
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Rakwal Randeep
Shibato Junko
Iwahashi Hitoshi
Jwa Nam-Soo
Kimura Shinzo
Nojima Kumie
Kim Yeon-Ki
Nahm Baek-Hie
Endo Satoru
Tanaka Kenichi
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Abstract
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The carbon-ion beam (CIB) generated by the heavy-ion medical accelerator in Chiba (HIMAC) was targeted to 7-day-old rice. Physiological parameters such as growth, and gene expression profiles were examined immediately after CIB irradiation. Dose-dependent growth suppression was seen three days post-irradiation (PI), and all the irradiated plants died by 15 days PI. Microarray (Agilent rice 22K) analysis of the plants immediately after irradiation (iai) revealed effects on gene expression at 270 Gy; 353 genes were up-regulated and 87 down-regulated. Exactly the same set of genes was affected at 90 Gy. Among the highly induced genes were genes involved in information storage and processing, cellular processes and signaling, and metabolism. RT-PCR analysis confirmed the microarray data.
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KEYWORD
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DNA Microarray, Early Gene Expression, Radiation, Rice, Stress Response
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