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KMID : 0578320080250020289
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Molecules and Cells
2008 Volume.25 No. 2 p.289 ~ p.293
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Repression of Transcriptional Activity of Estrogen Receptor alpha by a Cullin3/SPOP Ubiquitin E3 Ligase Complex
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Byun Boo-Hyeong
Jung Yun-Hwa
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Abstract
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The role of SPOP in the ubiquitination of ER alpha by the Cullin3-based E3 ubiquitin ligase complex was investigated. We showed that the N-terminal region of SPOP containing the MATH domain interacts with the AF-2 domain of ER alpha in cultured human embryonic 293 cells. SPOP was required for coimmunoprecipitation of ER alpha; with Cullin3. This is the first report of the essential role of SPOP in ERalpha ubiquitination by the Cullin3-based E3 ubiquitin ligase complex. We also demonstrated repression of the transactivation capability of ER alpha; in cultured mammalian cells.
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KEYWORD
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Cullin3-based E3 Ubiquitin Ligase Complex, ERα, SPOP, Ubiquitination
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