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KMID : 0578320080250020312
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Molecules and Cells
2008 Volume.25 No. 2 p.312 ~ p.316
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Flavanone 3beta-Hydroxylases from Rice: Key Enzymes for Favonol and Anthocyanin Biosynthesis
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Kim Jeong-Ho
Lim Yoong-Ho
Ahn Joong-Hoon
Kim Bong-Gyu
Lee Yoon-Jung
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Abstract
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Flavanone 3β-hydroxylases (F3H) are key enzymes in the synthesis of flavonol and anthocyanin. In this study, three F3H cDNAs from Oryza sativa (OsF3H-1 ~3) were cloned by RT-PCR and expressed in E. coli as gluthatione S-transferase (GST) fusion proteins. The purified recombinant OsF3Hs used flavanone, naringenin and eriodictyol as substrates. The reaction products with naringen and eriodictyol were determined by nuclear magnetic resonance spectroscopy to be dihydrokaempferol and taxifolin, respectively. OsF3H-1 had the highest enzymatic activity whereas the overall expression of OsF3H-2 was highest in all tissues except seeds. Flavanone 3β-hydroxylase could be a useful target for flavonoid metabolic engineering in rice.
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KEYWORD
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Flavanone 3β-Hydroxylase, Flavonoid, Oryza sativa
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