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KMID : 0578320080250040559
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Molecules and Cells
2008 Volume.25 No. 4 p.559 ~ p.565
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Isolation of CONSTANS as a TGA4/OBF4 Interacting Protein
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Song Young-Hun
Lim Chae-Oh
Lee Sang-Yeol
Kang Kyu-Young
Yun Dae-Jin
Song Na-Young
Shin Su-Young
Kim Hye-Jin
Hong Jong-Chan
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Abstract
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Members of the TGA family of basic domain/leucine zipper transcription factors regulate defense genes through physical interaction with NON-EXPRESSOR OF PR1 (NPR1). Of the seven TGA family members, TGA4/octopine synthase (ocs)-element-binding factor 4 (OBF4) is the least understood. Here we present evidence for a novel function of OBF4 as a regulator of flowering. We identified CONSTANS (CO), a positive regulator of floral induction, as an OBF4-interacting protein, in a yeast two-hybrid library screen. OBF4 interacts with the B-box region of CO. The abundance of OBF4 mRNA cycles with a 24 h rhythm under both long-day (LD) and short-day (SD) conditions, with significantly higher levels during the night than during the day. Electrophoretic mobility shift assays revealed that OBF4 binds to the promoter of the FLOWERING LOCUS T (FT) gene, a direct target of CO. We also found that, like CO and FT, an OBF4:GUS construct was prominently expressed in the vascular tissues of leaf, indicating that OBF4 can regulate FT expression through the formation of a protein complex with CO. Taken together, our results suggest that OBF4 may act as a link between defense responses and flowering.
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KEYWORD
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Circadian Clock, CONSTANS, Defense Response, FT, OBF4, SA, Stress
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