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KMID : 0578320080260040362
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Molecules and Cells
2008 Volume.26 No. 4 p.362 ~ p.367
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Identification of a Cryptic Type III Polyketide Synthase (1,3,6,8-Tetrahydroxynaphthalene Synthase) from Streptomyces peucetius ATCC 27952
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Ghimire Gopal Prasad
Oh Tae-Jin
Liou Kwangkyoung
Sohng Jae-Kyung
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Abstract
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We identified a 1,134-bp putative type III polyketide synthase from the sequence analysis of Streptomyces peucetius ATCC 27952, named Sp-RppA, which is characterized as 1,3,6,8-tetrahydroxynaphthalene synthase and shares 33% identity with SCO1206 from S. coelicolor A3(2) and 32% identity with RppA from S. griseus. The 1,3,6,8-tetrahydrox-ynaphthalene synthase is known to catalyze the sequential decarboxylative condensation, intramolecular cyclization, and aromatization of an oligoketide derived from five units of malonyl-CoA to give 1,3,6,8-tetrahydroxynaphthalene, which spontaneously oxidizes to form 2,5,7-trihydroxy-1,4-naphthoquinone (flaviolin). In this study, we report the in vivo expression and in vitro synthesis of flaviolin from purified gene product (Sp-RppA).
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KEYWORD
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1,3,6,8-tetrahydroxynaphthalene synthase, chalcone synthase, heterologous expression, Streptomyces peucetius
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