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KMID : 0578320080260040380
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Molecules and Cells
2008 Volume.26 No. 4 p.380 ~ p.386
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Identification of Osteogenic Purmorphamine Derivatives
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Lee Sung-Jin
Lee Hak-Kyo
Cho Sung-Yun
Choi Joong-Kwon
Shin Hea-Kyeong
Kwak Eun-Jung
Cho Mi-Ran
Kim Hye-Ryun
Kim Seung-Ryul
Kim Yong-Min
Park Kyoung-Jin
Choi Joong-Kook
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Abstract
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During embryonic and cancer development, the Hedgehog family of proteins, including Sonic Hedgehog, play an important role by relieving the inhibition of Smo by Ptc, thus activating the Smo signaling cascade. Recently, a purine compound, purmorphamine, has been reported to target the Hedgehog signaling pathway by interacting with Smo. Interestingly, both Sonic Hedgehog and purmorphamine were found to promote the osteogenic differentiation of mouse chondroprogenitor cells. However, there is insufficient information as to how the activation of this seemingly unrelated signaling pathway, either by Sonic Hedgehog or purmorphamine, contributes to osteogenesis. Using alkaline phosphatase assays, we screened 125 purmorphamine derivatives from the Korea Chemical Bank for effects on the differentiation of preosteoblast C2C12 cells. Here, we report that two purine derivatives modulate ALP activity as well as the expression of genes whose expression is known or suggested to be involved in osteogenesis.
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KEYWORD
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alkaline phosphatase assay, C2C12 preosteoblasts, osteogenesis, purmorphamine, Sonic hedgehog
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