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KMID : 0578320090270020243
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Molecules and Cells
2009 Volume.27 No. 2 p.243 ~ p.250
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HIF-1 alpha Dependent Gene Expression Program During the Nucleic Acid-Triggered Antiviral Innate Immune Responses
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Hong Sun-Woo
Yoo Jae-Wook
Kang Hye-Suk
Kim So-Youn
Lee Dong-ki
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Abstract
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Recent studies suggest a novel role of HIF-1??under non-hypoxic conditions, including antibacterial and antiviral innate immune responses. However, the identity of the pathogen-associated molecular pattern which triggers HIF-1??activation during the antiviral response remains to be identified. Here, we demonstrate that cellular administration of double-stranded nucleic acids, the molecular mimics of viral genomes, results in the induction of HIF-1??protein level as well as the increase in HIF-1??target gene expression. Whole-genome DNA microarray analysis revealed that double-stranded nucleic acid treatment triggers induction of a number of hypoxia-inducible genes, and induction of these genes are compromised upon siRNA-mediated HIF-1??knock-down. Interestingly, HIF-1??knock-down also resulted in down-regulation of a number of genes involved in antiviral innate immune responses. Our study demonstrates that HIF-1??activation upon nucleic acid-triggered antiviral innate immune responses plays an important role in regulation of genes involved in not only hypoxic response, but also immune response.
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KEYWORD
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Antiviral response, DNA microarray, dsDNA, dsRNA, HIF-1α, nnate immunity
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