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KMID : 0578320090270030329
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Molecules and Cells
2009 Volume.27 No. 3 p.329 ~ p.336
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Double Mutations in eIF4E and eIFiso4E Confer Recessive Resistance to Chilli Veinal Mottle Virus in Pepper
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Hwang Jee-Na
Li Jin-Jie
Liu Wing-Yee
An Song-Ji
Cho Hwa-Jin
Her Nam-Han
Yeam In-Hwa
Kim Do-Sun
Kang Byoung-Cheorl
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Abstract
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To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinal mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum empsey??containing an eIF4E mutation (pvr12) and C. annuum erennial??containing an eIFiso4E mutation (pvr6). C. annuum empsey??was susceptible and C. annuum erennial??was resistant to ChiVMV. All F1 plants showed resistance, and F2 individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five F2 and 329 F3 plants of 17 families were genotyped with pvr12 and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both pvr12 and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in eIF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of F2 plants revealed that all plants containing homozygous genotypes of both pvr12 and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of eIF4E and elFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.
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KEYWORD
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eIF4E, eIFiso4E, host factor, pepper, recessive virus resistance
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