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KMID : 0578320090270040403
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Molecules and Cells
2009 Volume.27 No. 4 p.403 ~ p.408
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Leukotriene B(4) Regulates Proliferation and Differentiation of Cultured Rat Myoblasts via the BLT1 Pathway
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Sun Ru
Ba Xueqing
Cui Lingling
Xue Yan
Zeng Xianlu
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Abstract
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Skeletal muscle regeneration is a highly orchestrated process initiated by activation of adult muscle satellite cells. Upon muscle injury, the inflammatory process is always accompanied by muscle regeneration. Leukotriene B4 is one of the essential inflammatory mediators. We isolated and cultured primary satellite cells. RT-PCR showed that myoblasts expressed mRNA for LTB4 receptors BLT1 and BLT2, and LTB4 promoted myoblast proliferation and fusion. Quantitative real-time PCR and immunoblotting showed that LTB4 treatment expedited the expression process of differentiation markers MyoD and M-cadherin. U-75302, a specific BLT1 inhibitor, but not LY2552833, a specific BLT2 inhibitor, blocked proliferation and differentiation of myoblasts induced by LTB4, which implies the involvement of the BLT1 pathway. Overall, the data suggest that LTB4 contributes to muscle regeneration by accelerating proliferation and differentiation of satellite cells.
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KEYWORD
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BLT1, BLT2, LTB4, M, cadherin, MyoD, satellite cells
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