KMID : 0578320090270040493
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Molecules and Cells 2009 Volume.27 No. 4 p.493 ~ p.0
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Backbone Resonance Assignment of a Proteoly-sis- Resistant Fragment in the Oxygen-Dependent Degradation Domain of the Hypoxia Inducible Factor 1 alpha
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Kim Do-Hyoung
Lee Si-Hyung Chi Seung-Wook Nam Ki-Hoon Han Kyou-Hoon
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Abstract
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Hypoxia-inducible factor 1¥á (HIF1¥á) is a transcription factor that plays a key role in the adaptation of cells to low oxygen stress and oxygen homeostasis. The oxygen-dependent degradation (ODD) domain of HIF1¥á responsible for the negative regulation of HIF1¥á in normoxia is intrinsically unfolded. Here, we carried out the backbone 1H, 15N, and 13C resonance assignment of a proteolysis-resistant fragment (residues 404?477) in the HIF1¥á ODD domain using NMR spectroscopy. About 98% (344/352) of all the 1HN, 15N, 13C¥á, 13C¥â, and 13CO resonances were unambiguously assigned. The results will be useful for further investigation of the structural and dynamic states of the HIF1¥á ODD domain and its interaction with binding partners.
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KEYWORD
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hypoxia, inducible factor 1¥á, NMR spectroscopy, oxygen, dependent degradation domain, resonance assignment
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