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KMID : 0578320090270050563
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Molecules and Cells
2009 Volume.27 No. 5 p.563 ~ p.570
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The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice
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Koo Sung-Cheol
Choi Man-Soo
Chun Hyun-Jin
Shin Dong-Bum
Park Bong-Soo
Kim Yul-Ho
Park Hyang-Mi
Seo Hak-Soo
Song Jong-Tae
Kang Kyu-Young
Yun Dae-Jin
Chung Woo-Sik
Cho Moo-Je
Kim Min-Chul
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Abstract
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We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.
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KEYWORD
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bacterial blight pathogen, CaM-binding protein, plant defense signaling, rice blast fungus, transcription factor
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