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KMID : 0578320090280040369
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Molecules and Cells
2009 Volume.28 No. 4 p.369 ~ p.373
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Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes
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Jeon Eu-Gene
Hyeon Jeong-Eun
Han Sung-Ok
Suh Dong-Jin
Suh Young-Woong
Kim Seoung-Wook
Song Kwang-Ho
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Abstract
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Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.
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KEYWORD
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beta-glucosidase, cellulose degradation, Clostridium thermocellum, endoglucanase, ethanol production, extracellular expression, Saccharomyces cerevisiae
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