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KMID : 0578320090280040397
Molecules and Cells
2009 Volume.28 No. 4 p.397 ~ p.401
Metabolic engineering of Escherichia coli for the biological synthesis of 7-O-xylosyl naringenin
Simkhada Dinesh

Kim Eui-Min
Lee Hei-Chan
Sohng Jae-Kyung
Abstract
Flavonoids are a group of polyphenolic compounds that have been recognized as important due to their physiological and pharmacological roles and their health benefits. Glycosylation of flavonoids has a wide range of effects on flavonoid solubility, stability, and bioavailability. We previously generated the E. coli BL21 (DE3) ¥Äpgi host by deleting the glucose-phosphate isomerase (Pgi) gene in E. coli BL21 (DE3). This host was further engineered for whole-cell biotransformation by integration of galU from E. coli K12, and expression of calS8 (UDP-glucose dehydrogenase) and calS9 (UDP-glucuronic acid decarboxylase) from Micromonospora echinospora spp. calichensis and arGt-4 (7-O-glycosyltransferase) from Arabidopsis thaliana to form E. coli (US89Gt-4), which is expected to produce glycosylated flavonoids. To test the designed system, the engineered host was fed with naringenin as a substrate, and naringenin 7-O-xyloside, a glycosylated naringenin product, was detected. Product was verified by HPLCLC/MS and ESI-MS/MS analyses. The reconstructed host can be applied for the production of various classes of glycosylated flavonoids.
KEYWORD
7-O-xylosyl naringenin, glycosylation, metabolic engineering, naringenin, UDP-D-xylose
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