|
KMID : 0578320100290020209
|
|
Molecules and Cells
2010 Volume.29 No. 2 p.209 ~ p.215
|
|
|
Melittin Suppresses PMA-Induced Tumor Cell Invasion by Inhibiting NF-κB and AP-1-Dependent MMP-9 Expression
|
|
Park Jung-Hyun
Jeong Yun-Jeong
Park Kwan-Kyu
Cho Hyun-Ji
Chung Il-Kyung
Min Kwan-Sik
Kim Mi-Hyun
Lee Kwang-Gill
Yeo Joo-Hong
Park Kwang-Kyun
Chang Young-Chae
|
|
|
Abstract
|
|
|
|
Matrix metalloproteinase-9 (MMP-9) plays an important role in the invasion and metastasis of cancer cells. In this study, we examined the inhibitory effect of bee venom (BV) and its major peptides, melittin and apamin, on PMA-induced invasion induced by MMP-9 expression in Caki-1 renal cancer cells. BV and melittin, but not apamin, significantly suppressed PMA-induced invasion by inhibiting MMP-9 expression in Caki-1 cells. Furthermore, as evidenced by MMP-9 promoter assays, melittin inhibited MMP-9 gene expression by blocking the PMA-stimulated activations of activator protein-1 (AP-1) and nuclear factor-kappa B (NF-κB). In addition, melittin suppressed the PMA-induced phosphorylations of ERK and JNK mitogen-activated protein kinases, upstream factors involved in Ap-1 and NF-κB. These results suggest that the suppression of MMP-9 expression contributes to the anti-tumor properties of melittin.
|
|
|
KEYWORD
|
|
|
bee venom, Caki-1 renal carcinoma cells, invasion, melittin, MMP-9
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|