|
KMID : 0578320100290040363
|
|
Molecules and Cells
2010 Volume.29 No. 4 p.363 ~ p.371
|
|
|
An Archaeal NADH Oxidase Causes Damage to Both Proteins and Nucleic Acids under Oxidative Stress
|
|
Baolei Jia
Lee Sang-Min
Bang P. Pham
Cho Yoon-Seung
Yang Jae-Kyung
Byeon Hee-Seop
Kim Jong-Cheol
Cheong Gang-Won
|
|
|
Abstract
|
|
|
|
ADH oxidases (NOXs) catalyze the two-electron reduction of oxygen to H2O2 or four-electron reduction of oxygen to H2O. In this report, we show that an NADH oxidase from Thermococcus profundus (NOXtp) displays two forms: a native dimeric protein under physiological conditions and an oxidized hexameric form under oxidative stress. Native NOXtp displays high NADH oxidase activity, and oxidized NOXtp can accelerate the aggregation of partially unfolded proteins. The aggregates formed by NOXtp have characteristics similar to β-amyloid and Lewy bodies in neuro-degenerative diseases, including an increase of β-sheet content. Oxidized NOXtp can also bind nucleic acids and cause their degradation by oxidizing NADH to produce H2O2. Furthermore, Escherichia coli cells expressing NOXtp are less viable than cells not expressing NOXtp after treat-ment with H2O2. As NOXtp shares similar features with eukaryotic cell death isozymes and life may have originated from hyperthermophiles, we suggest that NOXtp may be an ancestor of cell death proteins.
|
|
|
KEYWORD
|
|
|
cell death, dual function, NADH oxidase, protein aggregation, thermophilic archaeon
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|