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KMID : 0578320100290040407
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Molecules and Cells
2010 Volume.29 No. 4 p.407 ~ p.412
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Treponema denticola Suppresses Expression of Human β-Defensin-2 in Gingival Epithelial Cells through Inhibition of TNFα Production nd TLR2 Activation
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Shin Ji-Eun
Choi Young-Nim
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Abstract
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We previously reported that Treponema denticola, a peri-odontal pathogen, suppressed the expression of human β-defensins (HBDs) and IL-8 in human gingival epithelial cells. To clarify the receptor(s) involved in the suppres-sion of HBD-2, immortalized gingival epithelial (HOK-16B) cells were infected with live or heat-killed T. denticola for 24 h, and the expression of HBD-2 was examined by real-time RT-PCR. Live T. denticola, but not heat-killed bacteria, suppressed the expression of HBD-2 about 40%. Time courses of suppression revealed that T. denticola suppressed HBD-2 expression only at late time points, which was accompanied with the suppression of TNF αproduction. Neutralization of TNFα with an antibody abrogated the suppressive effect of T. denticola on HBD-2. Accordingly, heat-killed T. denticola did not suppress TNFα production. Knock-down of toll-like receptor (TLR) 2 via RNA interference reversed the suppressive effect of T. denticola on the expression of HBD-3, but not on the production of TNFα. Collectively, T. denticola suppresses the expression of HBD-2 in gingival epithelial cells by inhibiting the TLR2 axis and TNFα production, which may contribute to the pathogenesis of periodontitis by T. denticola.
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KEYWORD
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gingival epithelium, human beta-defensin-2, TLR2, TNFα, Treponema denticola
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