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KMID : 0578320100290050463
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Molecules and Cells
2010 Volume.29 No. 5 p.463 ~ p.469
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The Pro/Hel Region Is Indispensable for Packaging Non-Replicating Turnip Yellow Mosaic Virus RNA, but Not Replicating Viral RNA
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Shin Hyun-Il
Kim Hak-Yong
Cho Tae-Ju
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Abstract
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Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA. The genomic RNA has a tRNA-like structure (TLS) at the 3´-end. The 3´-TLS and hairpins in the 5´-untranslated region supposedly serve as packaging signals; however, recent studies have shown that they do not play a role in TYMV RNA packaging. In this study, we focused on packaging signals by examining a series of deletion mutants of TYMV. Analysis of encapsidated viral RNA after agroinfiltration of the deletion constructs into Nicotiana benthamiana showed that the mutant RNA lacking the protease (Pro)/helicase (Hel) region was not encapsidated by the coat proteins provided in trans, implicating that a packaging signal lies in the Pro/Hel region. Examination of two Pro-Hel- mutants showed that protein activity from the Pro/Hel domains was dis-pensable for the packaging of the non-replicating TYMV RNA. In contrast, the mutant TYMV RNA lacking the Pro/Hel region was efficiently encapsidated when the mutant TYMV was co-introduced with a wild-type TYMV, suggesting that packaging mechanisms might differ depending on whether the virus is replicating or not.
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KEYWORD
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plant RNA virus, Pro/Hel region, RNA packaging, TYMV
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