|
KMID : 0578320100290060567
|
|
Molecules and Cells
2010 Volume.29 No. 6 p.567 ~ p.574
|
|
|
A Cyclophilin A CPR1 Overexpression Enhances Stress Acquisition in Saccharomyces cerevisiae
|
|
Kim Il-Sup
Kim Hyun-Young
Shin Sun-Young
Kim Young-Saeng
Lee Dong-Hee
Park Kyung-Moc
Yoon Ho-Sung
|
|
|
Abstract
|
|
|
|
Cyclophilins are conserved cis-trans peptidyl-prolyl isom-erase that are implicated in protein folding and function as molecular chaperones. We found the expression of cyclophilin A, Cpr1, changes in response to exposure to yeast Saccharomyces cerevisiae to abiotic stress conditions. The effect of Cpr1 overexpression in stress responses was therefore examined. The CPR1 gene was cloned to the yeast expression vector pVTU260 under regulation of an endogenous alcohol dehy-drogenase (ADH) promoter. The overexpression of Cpr1 drastically increased cell viability of yeast in the presence of stress inducers, such as cadmium, cobalt, copper, hydrogen peroxide, tert-butyl hydro-peroxide (t-BOOH), and sodium dodecyl sulfate (SDS). The Cpr1 expression also enhanced the cell rescue program resulting in a variety of antioxidanr enzymes including thioredoxin system (particularly, thioredoxin peroxidase), metabolic enzymes (glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase), and molecular chaperones (Hsp104, Hsp90, Hsp60 and Hsp42). Thus, our study illustrates the importance of Cpr1 as a molecular chaperone that improves cellular stress respon-ses through collaborative relationships with other proteins when yeast cells are exposed to adverse conditions, and it also premises the improvement of yeast strains.
|
|
|
KEYWORD
|
|
|
cell rescue proteins, cyclophilin A, protein expression, stress tolerance, Saccharomyces cerevisiae
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|