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KMID : 0578320100300020143
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Molecules and Cells
2010 Volume.30 No. 2 p.143 ~ p.148
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Ankyrin Repeat-Rich Membrane Spanning/Kidins220 Protein Interacts with Mammalian Septin 5
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Park Han-Jeong
Park Hwan-Woo
Lee Shin-Jae
Juan Carlos Arevalo
Park Young-Seok
Lee Seung-Pyo
Paik Ki-Suk
Moses V. Chao
Chang Mi-Sook
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Abstract
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Neurotrophin receptors utilize specific adaptor proteins to activate signaling pathways involved in various neuronal functions, such as neurite outgrowth and cytoskeletal remodeling. The Ankyrin-Repeat Rich Mem-brane Spanning (ARMS)/kinase D-interacting substrate-220 kDa (Kidins220) serves as a unique downstream adaptor protein of Trk receptor tyrosine kinases. To gain insight into the role of ARMS/Kidins220, a yeast two-hybrid screen of a rat dorsal root ganglion library was performed using the C-terminal region of ARMS/Kidins220 as bait. The screen identified a mammalian septin, Septin 5 (Sept5), as an interacting protein. Co-immunoprecipitation using lysates from transiently transfected HEK-293 cells revealed the specific interaction between ARMS/Kidins220 and Sept5. Endogenous ARMS/Kidins220 and Sept5 proteins were co-localized in primary hippocampal neurons and were also predominantly expressed at the plasma membrane and in the tips of growing neurites in nerve growth factor-treated PC12 cells. Mapping of Sept5 domains important for ARMS/Kidins220 binding revealed a highly conserved N-terminal region of Sept5. The direct interaction between ARMS/Kidins220 and Sept5 suggests a possible role of ARMS/Kidins220 as a functional link between neurotrophin receptors and septins to mediate neurotrophin-induced intracellular signaling events, such as neurite outgrowth and cytoskeletal remodeling.
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KEYWORD
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adaptor protein, neurotrophin, receptor, septin, signal transduction
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