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KMID : 0578320100300040295
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Molecules and Cells
2010 Volume.30 No. 4 p.295 ~ p.301
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A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences
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Chung In-Hyuk
Yoo Hye-Sook
Eah Jae-Yong
Yoon Hyun-Kyu
Jung Jin-Wook
Kim Chang-Bae
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Abstract
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DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridiza-tion, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.
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KEYWORD
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birds, cytochrome c oxidase I, DNA barcoding, Korea, microarray, molecular probes
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