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KMID : 0578320100300040363
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Molecules and Cells
2010 Volume.30 No. 4 p.363 ~ p.368
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The RstB Sensor Acts on the PhoQ Sensor to Control Expression of PhoP-Regulated Genes
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Nam Dae-Sil
Choi Eun-Na
Kweon Dae-Hyuk
Shin Dong-Woo
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Abstract
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The PhoP response regulator and PhoQ sensor, which are encoded by the phoPQ operon, constitute the PhoP/PhoQ two-component system. Genome-wide tran-scription ana-lysis revealed that heterologous expression of the RstB protein, a sensor of the RstA/RstB two-component system, leads to enhanced transcription of PhoP-activated genes in wild-type Salmonella. We deter-mined that RstB-induc-tion increases the levels of phoP mRNA as well as PhoP protein, while lack of the phoPQ genes abolishes RstB-promoted transcription of the PhoP-regulated genes. This regulatory function of RstB did not require its enzymatic activities, and thus the truncated RstB protein containing only periplasmic and transmembrane regions was able to promote PhoP-activated transcription. The RstB protein appeared to target the PhoQ sensor rather than the PhoP response regulator because RstB-induction failed to enhance transcription of the PhoP-regulated genes in a strain maintaining the normal PhoP function, even without PhoQ.
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KEYWORD
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crosstalk, PhoP/PhoQ, RstB Sensor, Salmonella, two-component system
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