|
KMID : 0578320100300060563
|
|
Molecules and Cells
2010 Volume.30 No. 6 p.563 ~ p.567
|
|
|
Chemoattractant-Mediated Rap1 Activation Requires GPCR/G Proteins
|
|
Cha In-Jun
Lee Sung-H.
Jeon Taeck-J.
|
|
|
Abstract
|
|
|
|
Rap1 is rapidly activated upon chemoattractant stimula-tion and plays an important role in cell adhesion and cytoskeletal reorganization during chemotaxis. Here, we de-monstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell move-ment, is activated through the G-protein coupled receptors cAR1/cAR3 and Gα2 proteins in a way independent of the cGMP signaling pathway.
|
|
|
KEYWORD
|
|
|
chemotaxis, Dictyostelium, GPCR, Rap1, signal transduction
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|