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KMID : 0578320110310010025
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Molecules and Cells
2011 Volume.31 No. 1 p.25 ~ p.31
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The RNase Activity of Rice Probenazole-Induced Protein1 (PBZ1) Plays a Key Role in Cell Death in Plants
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Kim Sang-Gon
Kim Sun-Tae
Yiming Wang
Yu Seok
Choi In-Soo
Kim Yong-Chul
Kim Woo-Taek
Ganesh Kumar Agrawal
Randeep Rakwal
Kang Kyu-Young
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Abstract
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Cell death is an important process of plant responses to development and biotic/abiotic stresses. In rice plants, PBZ1, a PR10 family protein, has been shown to accumu-late in tissues undergoing cell death. However, the func-tion of PBZ1 in cell death remains yet to be demonstrated. Here, we report that exogenous recombinant PBZ1 protein induces cell death in rice suspension-cultured cells (SCCs) and also in leaves of Nicotiana tabacum in a dose-dependent manner. This finding was confirmed in vivo in transgenic Arabidopsis lines harboring the PBZ1 gene under the control of a dexamethasone (DEX)-inducible promoter. The DEX-treated leaves of transgenic Arabidopsis induced expression of PBZ1 at transcript and protein levels and showed cell death morphology. TUNEL analysis detected DNA fragmentation, a hallmark of programmed cell death, in rice SCCs treated with the PBZ1 protein. Recombinant PBZ1 protein also exhibited RNase activity and exhibited internalization inside BY-2 cells. Taken together, PBZ1 induces cell death not only in rice, but also in tobacco and Arabidopsis via its RNase activity inside the cell. PBZ1 could be used as a marker to understand the me-chanism by which PBZ1 confers the cell death morpholo-gy in rice and other model plants.
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KEYWORD
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dexamethasone, PBZ1, PR-10 protein family, programmed cell death, RNase activity, TUNEL
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