|
KMID : 0578320110310020191
|
|
Molecules and Cells
2011 Volume.31 No. 2 p.191 ~ p.197
|
|
|
Regulation of Sperm-Specific Proteins by IFE-1, a Germline-Specific Homolog of eIF4E, in C. elegans
|
|
Ichiro Kawasaki
Jeong Myung-Hwan
Shim Yhong-Hee
|
|
|
Abstract
|
|
|
|
IFE-1 is one of the five C. elegans homologs of eIF4E, which is the mRNA 5' cap-binding component of the translation initiation complex eIF4F. Depletion of IFE-1 causes defects in sperm, suggesting that IFE-1 regulates a subset of genes required for sperm functions. To further understand the molecular function of IFE-1, proteomic analysis was performed to search for sperm proteins that are down-regulated in ife-1(ok1978); fem-3(q20) mutants relative to the fem-3(q20) control. The fem-3(q20) mutant background was used because it only produces sperm at restrictive temperature. Total worm proteins were subjected to 2D-DIGE, and differentially expressed protein spots were further identified by MALDI-TOF mass spectrometry. Among the identified proteins, GSP-3 and Major Sperm Proteins (MSPs) were found to be significantly down-regulated in the ife-1(ok1978) mutant. Moreover, RNAi of gsp-3 caused an ife-1-like phenotype. These results suggest that IFE-1 is required for efficient expression of some sperm-specific proteins, and the fertilization defect of ife-1 mutant is caused mainly by a reduced level of GSP-3.
|
|
|
KEYWORD
|
|
|
Caenorhabditis elegans, eIF4E, IFE-1, proteomic analysis, sperm
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|