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KMID : 0578320110320010077
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Molecules and Cells
2011 Volume.32 No. 1 p.77 ~ p.82
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Regulation of Vascular Endothelial Growth Factor Signaling by miR-200b
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Choi Young-Chul
Yoon Se-Na
Jeong Yong-Su
Yoon Jae-Seung
Baek Kwang-Hee
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Abstract
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Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3?-untranslated region (3?-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-in-duced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.
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KEYWORD
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Flt-1, KDR, microRNA, miR-200b, VEGF, VEGF signaling
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