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KMID : 0578320110320020123
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Molecules and Cells
2011 Volume.32 No. 2 p.123 ~ p.132
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The Genome-Wide Expression Profile of 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose-Treated MDA-MB-231 Breast Cancer Cells: Molecular Target on Cancer Metabolism
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Yu Woo-Sik
Jeong Soo-Jin
Kim Ji-Hyun
Song Hyo-Sook
Kim Min-Seok
Ko Eun-Jung
Lee Hyo-Jeong
Khil Jae-Ho
Jang Hyeung-Jin
Kim Young-Chul
Bae Hyun-Su
Chen Chang-Yan
Kim Sung-Hoon
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Abstract
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1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphe-nolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the sig-nificant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes.
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KEYWORD
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cancer metabolism, MDA-MB-231, microarray, PGG, real-time PCR
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