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KMID : 0578320110320030227
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Molecules and Cells
2011 Volume.32 No. 3 p.227 ~ p.234
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FVE, an Arabidopsis Homologue of the Retinoblastoma-Associated Protein That Regulates Flowering Time and Cold Response, Binds to Chromatin as a Large Multiprotein Complex
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Jeon Jin
Kim Jung-Mook
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Abstract
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Some genetic studies indicate that plant homologues of proteins involved in chromatin modification and remode-ling in other organisms may regulate plant development. Previously, we described an Arabidopsis mutant with altered cold-responsive gene expression (acg1) displaying a late flowering phenotype, a null allele of fve. FVE is a homologue of the mammalian retinoblastoma-associated protein (RbAp), one component of a histone deacetylase (HDAC) complex involved in transcriptional repression, and has been shown to be involved in the deacetylation of the FLOWERING LOCUS C (FLC) chromatin encoding for a repressor of flowering. In an effort to gain insight into the biochemical functions of FVE, we overexpressed FVE tagged with the hemagglutinin (HA) and FLAG epitope at the N-terminus in acg1 mutants. The results of physiological and molecular analyses demonstrated that FVE over-expression in acg1 rescued the mutant phenotypes, in-cluding late flowering and alterations in floral pathway gene expression such as FLC, SUPPRESSOR OF OVER-EXPRESSION OF CO1 (SOC1), and FLOWERING LOCUS T (FT), and also super-induced cold-responsive reporter gene expression. The chromatin immunoprecipitation experiments revealed the amplification of specific DNA regions of FLC and COLD-REGULATED 15A (COR15A), indicating that FVE may bind to the FLC and COR15A chromatin. Gel-filtration chromatography and the immunoprecipitation of putative FVE complexes showed that FVE forms a protein complex of approximately 1.0 MDa. These results demonstrate that FVE may exist as a multiprotein complex, similar to the mammalian HDAC complex harboring RbAp, to regulate flowering time and cold response by associating with the FLC and COR chromatin.
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KEYWORD
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chromatin, flowering, FVE, histone deacetylase, retinoblastoma-associated protein
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