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KMID : 0578320110320030243
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Molecules and Cells
2011 Volume.32 No. 3 p.243 ~ p.249
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Resveratrol Alters microRNA Expression Profiles in A549 Human Non-Small Cell Lung Cancer Cells
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Bae Seung-Hee
Lee Eun-Mee
Cha Hwa-Jun
Kim Ka-Ram
Yoon Yeong-Min
Lee Hyun-Jin
Kim Jong-Ran
Kim Yu-Jeong
Lee Hong-Ghi
Jeung Hoi-Kyung
Min Yoo-Hong
An Sung-Kwan
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Abstract
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Resveratrol is a plant phenolic phytoalexin that has been reported to have antitumor properties in several types of cancers. In particular, several studies have suggested that resveratrol exerts antiproliferative effects against A549 human non-small cell lung cancer cells; however, its me-chanism of action remains incompletely understood. Deregulation of microRNAs (miRNAs), a class of small, non-coding, regulatory RNA molecules involved in gene expression, is strongly correlated with lung cancer. In this study, we demonstrated that resveratrol treatment altered miRNA expression in A549 cells. Using microarray analysis, we identified 71 miRNAs exhibiting greater than 2-fold expression changes in resveratrol-treated cells relative to their expression levels in untreated cells. Furthermore, we identified target genes related to apoptosis, cell cycle regulation, cell proliferation, and differentiation using a miRNA target-prediction program. In conclusion, our data demonstrate that resveratrol induces considerable changes in the miRNA expression profiles of A549 cells, suggesting a novel approach for studying the anticancer mechanisms of resveratrol.
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KEYWORD
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A549, human non-small cell lung cancer cells, microRNA, resveratrol
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