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KMID : 0578320110320040325
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Molecules and Cells
2011 Volume.32 No. 4 p.325 ~ p.331
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C6-Ceramide Enhances Phagocytic Activity of Kupffer Cells through the Production of Endogenous Ceramides
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Choi Jong-Min
Chu So-Jung
Ahn Kyong-Hoon
Kim Seok-Kyun
Ji Jung-Eun
Won Jong-Hoon
Kim Hyung-Chul
Back Moon-Jung
Kim Dae-Kyong
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Abstract
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Ceramide has been suggested to be not only a tumor-suppressive lipid but also a regulator of phagocytosis. We examined whether exogenous cell-permeable C6-ceramide enhances the phagocytic activity of Kupffer cells (KCs) and affects the level of cellular ceramides. Rat KCs were isolated by collagenase digestion and differential centrifugation, using Percoll system. Phagocytic activity was measured by FACS analysis after incubating KCs with fluorescence-conjugated latex beads, and the level of cellular ceramide was analyzed by liquid chromatography tandem-mass spectrometry (LC-MS/MS). In this study we found that permeable C6-ceramide increases the cellular levels of endogenous ceramides via a sphingosine-re-cycling pathway leading to enhanced phagocytosis by KCs.
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KEYWORD
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ceramide, kupffer cells, phagocytosis, sphingomyelin, sphingosine-recycle
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