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KMID : 0578320110320040337
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Molecules and Cells
2011 Volume.32 No. 4 p.337 ~ p.342
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Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)
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Chao Yang
Tuanjie Zhao
Deyue Yu
Junyi Gai
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Abstract
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Quantitative trait loci (QTLs) that control the tissue cul-ture response in soybean were detected by using 184 recombinant inbred lines (RILs) derived from two varieties: Kefeng No.1 and Nannong 1138-2. The molecular map consisting of 834 molecular markers using this population covered space 2307.83 cM of the genome throughout 24 linkage groups. The performance of tissue culture in soybean was evaluated by two indices: callus induction frequency (CIF) and somatic embryos initiation frequency (SEIF). They were expressed as the number of explants producing callus/ the number of total explants and the number of explants producing somatic embryos/ the number of total explants, respectively. The RIL lines showed continuous segregation for both indices. With the composite interval mapping (CIM) described in Windows QTL Cartographer Version 2.5, three quantitative trait loci (QTLs) were identified for the frequency of callus induction, on chromosomes B2 and D2, accounting for phenotypic variation from 5.84% to 16.60%; four QTLs on chromo-some G were detected for the frequency of somatic em-bryos initiation and explained the phenotypic variation from 7.79% to 14.16%. The information of new QTLs identified in the present study will contribute to genetic improvement of regeneration traits with marker-assisted selection (MAS) in soybean.
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KEYWORD
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quantitative trait loci, recombinant inbred line population, somatic embryogenesis, soybean (Glycine max (L.) Merr.), tissue culture response
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