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KMID : 0578320120330010043
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Molecules and Cells
2012 Volume.33 No. 1 p.43 ~ p.51
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Suppression of NS3 and MP Is Important for the Stable Inheritance of RNAi-Mediated Rice Stripe Virus (RSV) Resistance Obtained by Targeting the Fully Complementary RSV-CP Gene
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Park Hyang-Mi
Choi Man-Soo
Kwak Do-Yeon
Lee Bong-Choon
Lee Jong-Hee
Kim Myeong-Ki
Kim Yeon-Gyu
Shin Dong-Bum
Park Soon-Ki
Kim Yul-Ho
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Abstract
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Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi con-struct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown plan-thoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants.
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KEYWORD
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movement protein (MP), Oryza sativa L., rice stripe virus (RSV), RNAi, RSV coat protein, silencing suppressor (NS3), small brown planthop-per (SBPH)
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