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KMID : 0578320120330020189
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Molecules and Cells
2012 Volume.33 No. 2 p.189 ~ p.193
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A MAP Kinase Pathway Is Implicated in the Pseudohyphal Induction by Hydrogen Peroxide in Candica albicans
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Kavitha Srinivasa
Kim Ji-Hyun
Yee Su-Dog
Kim Wan-Kee
Choi Won-Ja
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Abstract
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Hydrogen peroxide (H2O2) functions as a ubiquitous intracellular messenger besides as an oxidative stress molecule. This dual role is based on the distinct cellular responses against different concentrations of H2O2. Previously, we demonstrated that both low (> 1 mM) and high (4-10 mM) doses of exogenous H2O2 induce filamentous growth with distinct cell morphology and growth rate in Candida albicans, suggesting the different transcription response. In this study, we revealed that the sub-toxic and toxic levels of H2O2 indeed induced pseudohyphae, but not true hyphae. Supporting this, several hyphae-specific genes that are expressed in true hyphae induced by serum were not detected in either sub-toxic or toxic H2O2 condition. A DNA microarray analysis was conducted to reveal the transcription profiles in cells treated with sub-toxic and toxic conditions of H2O2. Under the sub-toxic condition, a small number of genes involved in cell proliferation and metabolism were up-regulated, whereas a large number of genes were up-regulated in the toxic condition where the genes required for growth and proliferation were selectively restricted. For pseudohyphal induction by sub-toxic H2O2, Cek1 MAPK activating the transcription factor Cph1 was shown to be important. The absence of expression of several hyphae-specific genes known to be downstream targets of Cph1-signaling pathway for true hyphae formation suggests that the Cek1-mediated signaling pathway is not solely responsible for pseudohyphal formation by sub-toxic H2O2 and, but instead, complex networking pathway may exists by the activation of different regulators.
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KEYWORD
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Candida albicans, H2O2, intracellular messenger, signaling pathway, transcription profiling
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