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KMID : 0578320120330030277
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Molecules and Cells
2012 Volume.33 No. 3 p.277 ~ p.283
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Aberrant microRNAs Expression in CD133+/CD326+ Human Lung Adenocarcinoma Initiating Cells from A549
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Sheng Lin
Jian-guo Sun
Jing-bo Wu
Hai-xia Long
Cong-hui Zhu
Tong Xiang
Hu Ma
Zhong-quan Zhao
Quan Yao
An-mei Zhang
Bo Zhu
Zheng-tang Chen
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Abstract
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Increasing evidence demonstrates that miRNAs are in-volved in the dysregulation of tumor initiating cells (TICs) in various tumors. Due to a lack of definitive markers, cell sorting is not an ideal separation method for lung adeno-carcinoma initiating cells. In this study, we combined pa-clitaxel with serum-free medium cultivation (inverse-induc-tion) to enrich TICs from A549 cells, marked by CD133/ CD326, defined features of stemness. We next investigated aberrant microRNAs in this subpopulation compared to normal cells with miRNA microarray and found that 50 miRNAs exhibited a greater than 2-fold change in expres-sion. As further validation, 10 miRNAs were chosen to perform quantitative RT-PCR on the A549 cell line and primary samples. The results suggest that aberrant ex-pression of miRNAs such as miR-29ab, miR-183, miR-17-5p and miR-127-3P may play an important role in regulat-ing the bio-behavior of TICs.
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KEYWORD
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A549, microarray, microRNA, paclitaxel, tumor initiating cells
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