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KMID : 0578320120330030309
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Molecules and Cells
2012 Volume.33 No. 3 p.309 ~ p.316
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Identification of EBP50 as a Specific Biomarker for Carcinogens Via the Analysis of Mouse Lymphoma Cellular Proteome
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Lee Yoen-Jung
Choi In-Kwon
Sheen Yhun-Yhong
Park Sue-Nie
Kwon Ho-Jeong
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Abstract
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To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carci-nogen treatment compared with treatment with noncarci-nogens were selected. Of the identified proteins, we fo-cused on the candidate biomarker ERM-binding phosphoprotein 50 (EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.
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KEYWORD
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biomarker, carcinogen, EBP50, toxicoproteomics
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