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KMID : 0578320120330040343
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Molecules and Cells
2012 Volume.33 No. 4 p.343 ~ p.350
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Nicotinic Acetylcholine Receptor α7 and β4 Subunits Contribute Nicotine-Induced Apoptosis in Periodontal Ligament Stem Cells
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Kim So-Yeon
Kang Kyung-Lhi
Lee Jeong-Chae
Heo Jung-Sun
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Abstract
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Nicotine, a major component of cigarette smoking, is the important risk factor for the development of periodontal disease. However, the mechanisms that underlie the cytotoxicity of nicotine in human periodontal ligament stem cells (PDLSCs) are largely unknown. Thus, the purpose of this study was to determine the cytotoxic effect of nicotine by means of nicotinic acetylcholine receptor (nAChR) activation in PDLSCs. We first detected alpha7 and beta4 nAChRs in PDLSCs. The gene expressions of alpha7 and beta4 nAChR were increased by nicotine administration. Nicotine significantly decreased cell viability at a concentration higher than 10-5 M. DNA fragmentation was also detected at high doses of nicotine treatment. Moreover, the detection of sub G1 phase and TUNEL assay demonstrated that nicotine significantly induced apoptotic cell death at 10-2 M concentration. Western blot analysis confirmed that p53 proteins were phosphorylated by nicotine. Under various doses of nicotine, a decrease in the anti-apoptotic pro-tein Bcl-2, but an increase in p53 and cleaved caspase-3 protein levels, was detected in a dose-dependent manner. However, the apoptotic effect of nicotine was inhibited by the pretreatment of alpha-bungarotoxin, a selective alpha7 nAChR antagonist or mecamylamine, a non-selective nAChR antagonist. Finally, increases in the subG1 phase and DNA fragmentation by nicotine was attenuated by each nAChR antagonist. Collectively, the presence of alpha7 and beta4 nAChRs in PDLSCs supports a key role of nAChRs in the modulation of nicotine-induced apoptosis.
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KEYWORD
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apoptosis, nAChRs, nicotine, periodontal disease, periodontal ligament stem cells
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