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KMID : 0578320120340010053
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Molecules and Cells
2012 Volume.34 No. 1 p.53 ~ p.59
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Screening of Tissue-Specific Genes and Promoters in Tomato by Comparing Genome Wide Expression Profiles of Arabidopsis Orthologues
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Lim Chan-Ju
Lee Ha-Yeon
Kim Woong-Bom
Lee Bok-Sim
Kim Jung-Eun
Raza Ahmad
Kim Hyun-A
Yi So-Young
Hur Cheol-Goo
Kwon Suk-Yoon
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Abstract
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Constitutive overexpression of transgenes occasionally interferes with normal growth and developmental processes in plants. Thus, the development of tissue-specific promoters that drive transgene expression has become agriculturally important. To identify tomato tissue-specific promoters, tissue-specific genes were screened using a series of in silico-based and ex-perimental procedures, including genome-wide orthologue searches of tomato and Arabidopsis databases, isolation of tissue-specific candidates using an Arabidopsis microarray database, and validation of tissue specificity by reverse transcrip-tion-polymerase chain reaction (RT-PCR) analysis and promoter assay. Using these procedures, we found 311 tissue-specific candidate genes and validated 10 tissue-specific genes by RT-PCR. Among these identified genes, histochemical analysis of five isolated promoter::GUS transgenic tomato and Arabidopsis plants revealed that their promoters have different but distinct tissue-specific activities in anther, fruit, and root, respectively. Therefore, it appears these in silico-based screening approaches in addition to the identification of new tissue-specific genes and promoters will be helpful for the further development of tailored crop development.
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KEYWORD
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orthologue search, promoter, tissue-specific gene, tomato
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