|
KMID : 0578320120340030323
|
|
Molecules and Cells
2012 Volume.34 No. 3 p.323 ~ p.328
|
|
|
Involvement of the cAMP Response Element Binding Protein, CREB, and Cyclin D1 in LPA-Induced Proliferation of P19 Embryonic Carcinoma Cells
|
|
Kim Min-Jung
Sun Yuan-Jie
Yang Hai-Jie
Kim Nam-Ho
Jeon Sung-Ho
Huh Sung-Oh
|
|
|
Abstract
|
|
|
|
Lysophosphatidic acid (LPA) is a lipid growth factor that induces proliferation of fibroblasts by activating the cAMP response element binding protein (CREB). Here, we further investigated whether LPA induces proliferation of P19 cells, a line of pluripotent embryonic carcinoma cells. 5?-Bromo-2-deoxyuridine incorporation and cell viability assays showed that LPA stimulated proliferation of P19 cells. Immunoblot experiments with P19 cells revealed that the mitogen activated protein kinases, including p-ERK, p38, pAKT, glycogen synthase kinase 3?, and CREB were phosphorylated by treatment with 10 ?M LPA. LPA-indu-ced phosphorylation of CREB was efficiently blocked by U0126 and H89, inhibitors of the MAP kinases ERK1/2 and mitogen- and stress-activated protein kinase 1, respec-tively. Involvement of cyclin D1 in LPA-induced P19 cell proliferation was verified by immunoblot analysis in combination with pharmacological inhibitor treatment. Furthermore, LPA up-regulated CRE-harboring cyclin D1 promoter activity, suggesting that CREB and cyclin D1 play significant roles in LPA-induced proliferation of P19 embryonic carcinoma cells.
|
|
|
KEYWORD
|
|
|
cAMP response element binding protein (CREB), cyclin D1, lysophosphatidic acid, P19 embryonic carcinoma cell, proliferation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|